Human HMBS(Hydroxymethylbilane Synthase) ELISA Kit

Human HMBS(Hydroxymethylbilane Synthase) ELISA Kit

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    Human Hydroxymethylbilane Synthase (HMBS) ELISA Kit
    RDR-HMBS-Hu-48Tests 48 Tests
    EUR 544

    Human Hydroxymethylbilane Synthase (HMBS) ELISA Kit
    RDR-HMBS-Hu-96Tests 96 Tests
    EUR 756

    Human Hydroxymethylbilane Synthase (HMBS) ELISA Kit
    SEE673Hu-10x96wellstestplate 10x96-wells test plate
    EUR 4731.5
    Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hydroxymethylbilane Synthase (HMBS) in tissue homogenates, cell lysates and other biological fluids.

    Human Hydroxymethylbilane Synthase (HMBS) ELISA Kit
    SEE673Hu-1x48wellstestplate 1x48-wells test plate
    EUR 477.3
    Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hydroxymethylbilane Synthase (HMBS) in tissue homogenates, cell lysates and other biological fluids.

    Human Hydroxymethylbilane Synthase (HMBS) ELISA Kit
    SEE673Hu-1x96wellstestplate 1x96-wells test plate
    EUR 639
    Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hydroxymethylbilane Synthase (HMBS) in tissue homogenates, cell lysates and other biological fluids.

    Human Hydroxymethylbilane Synthase (HMBS) ELISA Kit
    SEE673Hu-5x96wellstestplate 5x96-wells test plate
    EUR 2575.5
    Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Hydroxymethylbilane Synthase (HMBS) in tissue homogenates, cell lysates and other biological fluids.

    Human Hydroxymethylbilane Synthase (HMBS) ELISA Kit
    4-SEE673Hu
    • EUR 4782.00
    • EUR 2526.00
    • EUR 640.00
    • 10 plates of 96 wells
    • 5 plates of 96 wells
    • 1 plate of 96 wells
    Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Hydroxymethylbilane Synthase (HMBS) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

    Human Hydroxymethylbilane Synthase (HMBS) ELISA Kit
    20-abx151823
    • EUR 7378.00
    • EUR 3933.00
    • EUR 911.00
    • 10 × 96 tests
    • 5 × 96 tests
    • 96 tests

    Human Hydroxymethylbilane Synthase ELISA Kit (HMBS)
    RK01572 96 Tests
    EUR 521

    Recombinant Hydroxymethylbilane Synthase (HMBS)
    4-RPE673Hu01
    • EUR 467.36
    • EUR 228.00
    • EUR 1477.60
    • EUR 559.20
    • EUR 1018.40
    • EUR 376.00
    • EUR 3544.00
    • 100 ug
    • 10ug
    • 1 mg
    • 200 ug
    • 500 ug
    • 50ug
    • 5 mg
    Description: Recombinant Human Hydroxymethylbilane Synthase expressed in: E.coli

    Hydroxymethylbilane Synthase (HMBS) Antibody
    20-abx129004
    • EUR 425.00
    • EUR 133.00
    • EUR 1205.00
    • EUR 578.00
    • EUR 328.00
    • 100 ug
    • 10 ug
    • 1 mg
    • 200 ug
    • 50 ug

    Hydroxymethylbilane Synthase (HMBS) Antibody
    20-abx113081
    • EUR 732.00
    • EUR 398.00
    • 150 ul
    • 50 ul

    Hydroxymethylbilane Synthase (HMBS) Antibody
    20-abx172852
    • EUR 857.00
    • EUR 439.00
    • 1 mg
    • 200 ug

    Human Hydroxymethylbilane Synthase (HMBS) Protein
    20-abx168094
    • EUR 648.00
    • EUR 272.00
    • EUR 1998.00
    • EUR 773.00
    • EUR 467.00
    • 100 ug
    • 10 ug
    • 1 mg
    • 200 ug
    • 50 ug

    Human Hydroxymethylbilane Synthase (HMBS) CLIA Kit
    20-abx494686
    • EUR 7973.00
    • EUR 4246.00
    • EUR 981.00
    • 10 × 96 tests
    • 5 × 96 tests
    • 96 tests

    ELISA kit for Human HMBS (Hydroxymethylbilane Synthase)
    ELK4111 1 plate of 96 wells
    EUR 432
    Description: A sandwich ELISA kit for detection of Hydroxymethylbilane Synthase from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

    HMBS Hydroxymethylbilane Synthase Human Recombinant Protein
    PROTP08397 Regular: 20ug
    EUR 317
    Description: HMBS Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 385 amino acids (1-361) and having a molecular mass of 41.9kDa.;HMBS is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Hydroxymethylbilane Synthase (HMBS) Polyclonal Antibody (Human, Pig)
    4-PAE673Hu01
    • EUR 247.00
    • EUR 2510.00
    • EUR 625.00
    • EUR 310.00
    • EUR 214.00
    • 100ul
    • 10ml
    • 1ml
    • 200ul
    • 20ul
    Description: A Rabbit polyclonal antibody against Human, Pig Hydroxymethylbilane Synthase (HMBS)

    Hydroxymethylbilane Synthase (HMBS) Polyclonal Antibody (Human, Pig), APC
    4-PAE673Hu01-APC
    • EUR 345.00
    • EUR 3275.00
    • EUR 912.00
    • EUR 440.00
    • EUR 219.00
    • 100ul
    • 10ml
    • 1ml
    • 200ul
    • 20ul
    Description: A Rabbit polyclonal antibody against Human, Pig Hydroxymethylbilane Synthase (HMBS). This antibody is labeled with APC.

    Hydroxymethylbilane Synthase (HMBS) Polyclonal Antibody (Human, Pig), Biotinylated
    4-PAE673Hu01-Biotin
    • EUR 311.00
    • EUR 2460.00
    • EUR 727.00
    • EUR 381.00
    • EUR 219.00
    • 100ul
    • 10ml
    • 1ml
    • 200ul
    • 20ul
    Description: A Rabbit polyclonal antibody against Human, Pig Hydroxymethylbilane Synthase (HMBS). This antibody is labeled with Biotin.

    Hydroxymethylbilane Synthase (HMBS) Polyclonal Antibody (Human, Pig), Cy3
    4-PAE673Hu01-Cy3
    • EUR 419.00
    • EUR 4325.00
    • EUR 1175.00
    • EUR 545.00
    • EUR 251.00
    • 100ul
    • 10ml
    • 1ml
    • 200ul
    • 20ul
    Description: A Rabbit polyclonal antibody against Human, Pig Hydroxymethylbilane Synthase (HMBS). This antibody is labeled with Cy3.

    Hydroxymethylbilane Synthase (HMBS) Polyclonal Antibody (Human, Pig), FITC
    4-PAE673Hu01-FITC
    • EUR 296.00
    • EUR 2640.00
    • EUR 750.00
    • EUR 372.00
    • EUR 195.00
    • 100ul
    • 10ml
    • 1ml
    • 200ul
    • 20ul
    Description: A Rabbit polyclonal antibody against Human, Pig Hydroxymethylbilane Synthase (HMBS). This antibody is labeled with FITC.

    Hydroxymethylbilane Synthase (HMBS) Polyclonal Antibody (Human, Pig), HRP
    4-PAE673Hu01-HRP
    • EUR 316.00
    • EUR 2855.00
    • EUR 807.00
    • EUR 398.00
    • EUR 206.00
    • 100ul
    • 10ml
    • 1ml
    • 200ul
    • 20ul
    Description: A Rabbit polyclonal antibody against Human, Pig Hydroxymethylbilane Synthase (HMBS). This antibody is labeled with HRP.

    Hydroxymethylbilane Synthase (HMBS) Polyclonal Antibody (Human, Pig), PE
    4-PAE673Hu01-PE
    • EUR 296.00
    • EUR 2640.00
    • EUR 750.00
    • EUR 372.00
    • EUR 195.00
    • 100ul
    • 10ml
    • 1ml
    • 200ul
    • 20ul
    Description: A Rabbit polyclonal antibody against Human, Pig Hydroxymethylbilane Synthase (HMBS). This antibody is labeled with PE.

    Hydroxymethylbilane Synthase (HMBS) Polyclonal Antibody (Human, Pig), APC-Cy7
    4-PAE673Hu01-APC-Cy7
    • EUR 571.00
    • EUR 6430.00
    • EUR 1705.00
    • EUR 760.00
    • EUR 319.00
    • 100ul
    • 10ml
    • 1ml
    • 200ul
    • 20ul
    Description: A Rabbit polyclonal antibody against Human, Pig Hydroxymethylbilane Synthase (HMBS). This antibody is labeled with APC-Cy7.

    Hydroxymethylbilane Synthase (Recombinant)
    20-abx073331
    • EUR 3418.00
    • EUR 328.00
    • EUR 230.00
    • 1 mg
    • 20 ug
    • 5 ug

    Hmbs/ Rat Hmbs ELISA Kit
    ELI-30905r 96 Tests
    EUR 886

    HMBS ELISA KIT|Human
    EF010156 96 Tests
    EUR 689

    HMBS ELISA Kit (Human) (OKEH02521)
    OKEH02521 96 Wells
    EUR 831
    Description: Description of target: This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.327 ng/mL

    HMBS ELISA Kit (Human) (OKCD08681)
    OKCD08681 96 Wells
    EUR 975
    Description: Description of target: HMBS is a member of the hydroxymethylbilane synthase superfamily. It is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria.This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.26ng/mL

    HMBS ELISA Kit (Human) (OKAN06214)
    OKAN06214 96 Wells
    EUR 792
    Description: Description of target: This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.26 ng/mL

    Human HMBS/ Porphobilinogen deaminase ELISA Kit
    E1138Hu 1 Kit
    EUR 605

    Human Porphobilinogen deaminase (HMBS) ELISA Kit
    abx573587-96tests 96 tests
    EUR 739

    Human Porphobilinogen deaminase, HMBS ELISA KIT
    ELI-48602h 96 Tests
    EUR 824

    Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed
    ELISA-1 1
    EUR 202

    Human HMBS Antibody
    35685-05111 150 ug
    EUR 261

    HMBS Antibody
    1-CSB-PA010524GA01HU
    • EUR 597.00
    • EUR 333.00
    • 150ul
    • 50ul
    Description: A polyclonal antibody against HMBS. Recognizes HMBS from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF

    HMBS Antibody
    CSB-PA010524KA01HU-
    EUR 335
    Description: A polyclonal antibody against HMBS. Recognizes HMBS from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

    HMBS Antibody
    CSB-PA010524KA01HU-100ul 100ul
    EUR 389
    Description: A polyclonal antibody against HMBS. Recognizes HMBS from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

    HMBS Antibody
    1-CSB-PA010524LA01HU
    • EUR 317.00
    • EUR 335.00
    • 100ug
    • 50ug
    Description: A polyclonal antibody against HMBS. Recognizes HMBS from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

    HMBS Antibody
    32430-100ul 100ul
    EUR 252

    HMBS siRNA
    20-abx919588
    • EUR 551.00
    • EUR 732.00
    • 15 nmol
    • 30 nmol

    HMBS siRNA
    20-abx919589
    • EUR 551.00
    • EUR 732.00
    • 15 nmol
    • 30 nmol

    HMBS Antibody
    49010-100ul 100ul
    EUR 333

    HMBS Antibody
    49010-50ul 50ul
    EUR 239

    HMBS Antibody
    ABD6611 100 ug
    EUR 438

    HMBS Antibody
    DF6611 200ul
    EUR 304
    Description: HMBS Antibody detects endogenous levels of total HMBS.

    HMBS antibody
    70R-17754 50 ul
    EUR 435
    Description: Rabbit polyclonal HMBS antibody

    HMBS antibody
    70R-3343 50 ug
    EUR 467
    Description: Rabbit polyclonal HMBS antibody raised against the N terminal of HMBS

    HMBS antibody
    70R-3585 50 ug
    EUR 467
    Description: Rabbit polyclonal HMBS antibody raised against the middle region of HMBS

    pOTB7-HMBS
    PVT18460 2 ug
    EUR 231

    anti-HMBS
    YF-PA12376 50 ul
    EUR 363
    Description: Mouse polyclonal to HMBS

    anti-HMBS
    YF-PA12377 100 ug
    EUR 403
    Description: Rabbit polyclonal to HMBS

    Mouse Hmbs/ Porphobilinogen deaminase ELISA Kit
    E0677Mo 1 Kit
    EUR 632

    Mouse Porphobilinogen deaminase (HMBS) ELISA Kit
    abx555617-96tests 96 tests
    EUR 739

    Rat Porphobilinogen deaminase (HMBS) ELISA Kit
    abx555711-96tests 96 tests
    EUR 739

    Cow Porphobilinogen deaminase (HMBS) ELISA Kit
    abx555776-96tests 96 tests
    EUR 911

    Bovine Porphobilinogen deaminase, HMBS ELISA KIT
    ELI-43928b 96 Tests
    EUR 928

    Mouse Porphobilinogen deaminase, Hmbs ELISA KIT
    ELI-30904m 96 Tests
    EUR 865

    Human HMBS shRNA Plasmid
    20-abx952146
    • EUR 801.00
    • EUR 1121.00
    • 150 µg
    • 300 µg

    HMBS Recombinant Protein (Human)
    RP014953 100 ug Ask for price

    HMBS cloning plasmid
    CSB-CL010524HU-10ug 10ug
    EUR 233
    Description: A cloning plasmid for the HMBS gene.

    Anti-HMBS antibody
    STJ24034 100 µl
    EUR 277
    Description: This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.

    Anti-HMBS Antibody
    A01506-1 100ug/vial
    EUR 294

    HMBS Rabbit mAb
    A11701-100ul 100 ul
    EUR 410

    HMBS Rabbit mAb
    A11701-200ul 200 ul
    EUR 571

    HMBS Rabbit mAb
    A11701-20ul 20 ul
    EUR 221

    HMBS Rabbit mAb
    A11701-50ul 50 ul
    EUR 287

    HMBS Rabbit pAb
    A1777-100ul 100 ul
    EUR 308

    HMBS Rabbit pAb
    A1777-200ul 200 ul
    EUR 459

    HMBS Rabbit pAb
    A1777-20ul 20 ul
    EUR 183

    HMBS Rabbit pAb
    A1777-50ul 50 ul
    EUR 223

    HMBS Conjugated Antibody
    C32430 100ul
    EUR 397

    anti- HMBS antibody
    FNab03918 100µg
    EUR 505.25
    Description: Antibody raised against HMBS

    HMBS Conjugated Antibody
    C49010 100ul
    EUR 397

    HMBS Blocking Peptide
    DF6611-BP 1mg
    EUR 195

    HMBS Blocking Peptide
    33R-6388 100 ug
    EUR 180
    Description: A synthetic peptide for use as a blocking control in assays to test for specificity of HMBS antibody, catalog no. 70R-3343

    HMBS Blocking Peptide
    33R-8825 100 ug
    EUR 180
    Description: A synthetic peptide for use as a blocking control in assays to test for specificity of HMBS antibody, catalog no. 70R-3585

    Anti-HMBS antibody
    PAab03918 100 ug
    EUR 355

    Anti-HMBS (3E8)
    YF-MA13480 100 ug
    EUR 363
    Description: Mouse monoclonal to HMBS

    Anti-HMBS (2B12)
    YF-MA13481 100 ug
    EUR 363
    Description: Mouse monoclonal to HMBS

    HMBS Recombinant Protein
    91-696 0.05 mg
    EUR 684.5
    Description: Porphobilinogen Deaminase (HMBS) is a member of the HMBS family. PBGD is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. HMBS is involved in the production of heme, which is important for all of the body's organs, although it is most abundant in the blood, bone marrow, and liver. In addition, Heme is an essential component of iron-containing proteins called hemoproteins, including hemoglobin. Defects in PBGD are the cause of acute intermittent porphyria.

    Human HMBS Antibody (Biotin Conjugate)
    35685-05121 150 ug
    EUR 369

    HMBS ORF Vector (Human) (pORF)
    ORF004985 1.0 ug DNA
    EUR 95

    Frit Kit
    FRIT-KIT 1each
    EUR 124
    Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

    Human Cardiolipin Synthase ELISA kit
    E01C0634-192T 192 tests
    EUR 1270
    Description: A competitive ELISA for quantitative measurement of Human Cardiolipin Synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

    Human Cardiolipin Synthase ELISA kit
    E01C0634-48 1 plate of 48 wells
    EUR 520
    Description: A competitive ELISA for quantitative measurement of Human Cardiolipin Synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

    Human Cardiolipin Synthase ELISA kit
    E01C0634-96 1 plate of 96 wells
    EUR 685
    Description: A competitive ELISA for quantitative measurement of Human Cardiolipin Synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

    Human Citrate Synthase ELISA kit
    E01C0830-192T 192 tests
    EUR 1270
    Description: A competitive ELISA for quantitative measurement of Human Citrate Synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

    Human Citrate Synthase ELISA kit
    E01C0830-48 1 plate of 48 wells
    EUR 520
    Description: A competitive ELISA for quantitative measurement of Human Citrate Synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

    Human Citrate Synthase ELISA kit
    E01C0830-96 1 plate of 96 wells
    EUR 685
    Description: A competitive ELISA for quantitative measurement of Human Citrate Synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

    Human Thromboxane synthase ELISA kit
    E01T0553-192T 192 tests
    EUR 1270
    Description: A sandwich ELISA for quantitative measurement of Human Thromboxane synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

    Human Thromboxane synthase ELISA kit
    E01T0553-48 1 plate of 48 wells
    EUR 520
    Description: A sandwich ELISA for quantitative measurement of Human Thromboxane synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

    Human Thromboxane synthase ELISA kit
    E01T0553-96 1 plate of 96 wells
    EUR 685
    Description: A sandwich ELISA for quantitative measurement of Human Thromboxane synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

    Column Packing Kit
    PACK-KIT 1pack
    EUR 1035
    Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

    HMBS Antibody, HRP conjugated
    1-CSB-PA010524LB01HU
    • EUR 317.00
    • EUR 335.00
    • 100ug
    • 50ug
    Description: A polyclonal antibody against HMBS. Recognizes HMBS from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

    HMBS Antibody, FITC conjugated
    1-CSB-PA010524LC01HU
    • EUR 317.00
    • EUR 335.00
    • 100ug
    • 50ug
    Description: A polyclonal antibody against HMBS. Recognizes HMBS from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

    HMBS Antibody, Biotin conjugated
    1-CSB-PA010524LD01HU
    • EUR 317.00
    • EUR 335.00
    • 100ug
    • 50ug
    Description: A polyclonal antibody against HMBS. Recognizes HMBS from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

    Mouse HMBS shRNA Plasmid
    20-abx970816
    • EUR 801.00
    • EUR 1121.00
    • 150 µg
    • 300 µg

    Porphobilinogen Deaminase (HMBS) Antibody
    abx117159-100ug 100 ug
    EUR 467

    Porphobilinogen Deaminase (HMBS) Antibody
    20-abx001474
    • EUR 411.00
    • EUR 592.00
    • EUR 182.00
    • EUR 314.00
    • 100 ul
    • 200 ul
    • 20 ul
    • 50 ul

    Porphobilinogen Deaminase (HMBS) Antibody
    abx038138-100ug 100 ug
    EUR 391

    Porphobilinogen Deaminase (HMBS) Antibody
    abx145984-100ug 100 ug
    EUR 391

    Porphobilinogen Deaminase (HMBS) Antibody
    abx432804-200ul 200 ul
    EUR 384

    Human HMBS(Hydroxymethylbilane Synthase) ELISA Kit